Protective efficacy of Streptococcus iniae derived enolase against Streptococcal infection in a zebrafish model

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• 作者：Juver D. Membrebe^a ; ^b ; Nam-Kyung Yoon^c ; Minhee Hong^c ; Jeongsoo Lee^d ; ^f ; Hongweon Lee^c ; ^e ; Kyoungmoon Park^g ; Sin-hye Seo^b ; Injoong Yoon^b ; Sungsik Yoo^b ; Yeu-Chun Kim^a ; ^{dohnanyi@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Jungoh Ahn^c ; ^e ; ^{ahnjo@kribb.re.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：α-Enolase ; Streptococcus iniae ; Streptococcus parauberis ; Subunit vaccine ; Zebrafish
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：170
• 期：Complete
• 页码：25-29
• 全文大小：570 K

文摘

Enolase (ENO) is one of the surface-exposed proteins of Streptococcus iniae, which previously had been identified as a plasminogen-binding protein. In this study, ENO was evaluated to induce cross-protective immunity against S. iniae and Streptococcus parauberis which are major pathogens causing streptococcosis in fish. Immunoblot analysis shows that S. iniae recombinant ENO (rENO) produced in Escherichia coli was cross-reactive with antisera against S. iniae, and S. parauberis serotype I and II. In the challenge experiment of streptococcal infection after vaccination in zebrafish, rENO elicited a similar protection with a whole cell bacterin against S. iniae and S. parauberis, which suggests its feasibility as an efficient vaccine against streptococcosis.