Establishment of a 2-week canine skin organ culture model and its pharmacological modulation by epidermal growth factor and dexamethasone

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• 作者：Francesca Abramo^a ; ^{francesca.abramo@unipi.it" class="auth_mail" title="E-mail the corresponding author} ; Andrea Pirone^a ; ^{andrea.pirone@unipi.it" class="auth_mail" title="E-mail the corresponding author} ; Carla Lenzi^a ; ^{carla.lenzi@unipi.it" class="auth_mail" title="E-mail the corresponding author} ; Iacopo Vannozzi^a ; ^{iacopo.vannozzi@unipi.it" class="auth_mail" title="E-mail the corresponding author} ; Maria Federica della Valle^b ; ^{fb@innovet.it" class="auth_mail" title="E-mail the corresponding author} ; Vincenzo Miragliotta^a ; ^{vincenzo.miragliotta@unipi.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dog ; Skin organ culture ; Epidermal growth factor ; Dexamethasone
• 刊名：Annals of Anatomy - Anatomischer Anzeiger
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：207
• 期：Complete
• 页码：109-117
• 全文大小：2332 K

文摘

Although canine skin models are already available as either monocellular or organotypic cultures, they only partly recapitulate normal skin morphological features and function. The objective of this study was to establish a canine serum-free skin organ culture model and verify whether dexamethasone could rescue epidermal growth factor-induced changes. The study of morphological changes as a response to pharmacological substances may indeed help to investigate skin physiology and pathology.

Normal skin was obtained from five client-owned dogs subjected to surgical procedures unrelated to dermatological conditions. Two experimental designs were performed: (i) two-week viability of the skin culture; (ii) dexamethasone (DMS) inhibition of epidermal growth factor (EGF)-induced effects. Serum-free submerged organ cultures were established in Williams’ E medium supplemented with penicillin-streptomycin, insulin, hydrocortisone and l-glutamine.

General morphological features of skin anatomical structures were well maintained up to day 14, scattered pyknotic nuclei were visible in the epidermis from day 7. Normal keratinocyte differentiation was confirmed by cytokeratin (K) 10, K14 and loricrin immunostaining. Epidermal thickness did not decrease throughout the study. A decrease in keratinocyte proliferation was observed at day 7 and 14. Treatment with EGF induced both keratinocyte proliferation and thickening of the epidermis; both responses were counteracted by DMS. Treatment with EGF increased the length of epithelial tongues at the edge of the skin explants; this effect was further enhanced by DMS supplementation.

Our findings demonstrate the potential use of a full-thickness canine skin organ culture model for the study of skin physiology and pharmacological response to exogenous compounds, especially in the field of re-epithelialisation and keratinization disorders.