Enzymatic methods for the determination of pollution in seawater using salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius
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- 作者：Natalie I. Menzorova ; Alex ; ra V. Seitkalieva ; Valer褍 A. Rasskazov
- 关键词：Alkaline phosphatase (AP) ; Eggs of sea urchin ; Strongylocentrotus intermedius ; Phosphatase inhibition assay ; Pollution monitoring ; Japan Sea
- 刊名：Marine Pollution Bulletin
- 出版年：15 February, 2014
- 年：2014
- 卷：79
- 期：1-2
- 页码：188-195
- 全文大小：651 K
文摘
A new salt resistant alkaline phosphatase from eggs of the sea urchin Strongylocentrotus intermedius (StAP) has been shown to have a unique property to hydrolyze substrate in seawater without loss of enzymatic activity. The enzyme has pH optimum at 8.0-8.5. Model experiments showed various concentrations of copper, zinc, cadmium and lead added to seawater or a standard buffer mixture to inhibit completely the enzyme activity at the concentrations of 15-150 渭g/l. StAP sensitivity to the presence in seawater of metals, pesticides, detergents and oil products appears to be considerably less. Samples of seawater taken from aquatic areas of the Troitsy Bay of the Peter the Great Bay, Japan Sea have been shown to inhibit the enzyme activity; the same was shown for the samples of fresh waters. The phosphatase inhibition assay developed proved to be highly sensitive, technically easy-to use allowing to test a great number of samples.