P-1262 - Aging and N-methyl-D-aspartate receptor subunits - nitric oxide synthases pathways in Nogo-A deficient rats

详细信息    查看全文

• 作者：Z. Kristofikova¹ ; M. Vrajova¹ ; D. Ripova¹ ; A. Stuchlik¹ ; K. Vales¹ ; T. Petrasek¹ ; D. Bartsch² ; ³
• 刊名：European Psychiatry
• 出版年：2012
• 出版时间：2012
• 年：2012
• 卷：27
• 期：supp_S1
• 页码：1
• 全文大小：66 K

文摘

Introduction

It seems that schizophrenia may arise from abnormal neurodevelopment due to aberrant neuron formation and migration and that deletion of Nogo-A (a myelin associated inhibitor of regenerative fiber growth) may lead to schizophrenia-like abnormalities in animal model. Our previous studies reported a high sensitivity of lateral analyses to reveal subtle links and recommended to apply them to validate animal models of diseases accompanied by alterations in brain asymmetry.

Methods

Expressions of N-methyl-D-aspartate receptor subunits (NR1, NR2A, NR2B) and activities of subunits-associated synthases of nitric oxide (nNOS, eNOS, iNOS) were evaluated in the right and left cortex of young or old Nogo-A deficient rats.

Results

In young controls, no marked laterality was observed in subunits/synthases excepting iNOS but correlation analyses supported links among some subunits, synthases or subunits - synthases. Drops in NR1 (bilaterally) and in NR2B (in the right side) or asymmetrical alterations in NR1 - nNOS pathway were observed in old controls. In young Nogo-A deficient rats, the increase in iNOS (in the left side) and the disruption in left NR1 - right nNOS or in right NR2A - right eNOS pathways were found. And finally in old Nogo-A deficient animals, we observed the increase in NR1 (bilaterally) and in positive correlation between right eNOS - right iNOS.

Conclusion

Although some changes in subunits/synthases observed in people with schizophrenia were not found in Nogo-A deficient rats, some alterations in laterality support the validity of this model.

Acknowledgements

The study was supported by MSMT (1M0517) and MZCR (MZ0PCP2005) projects.