The Transcriptional and Functional Properties of Mouse Epiblast Stem Cells Resemble the Anterior Primitive Streak

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• 作者：Yoji Kojima ; Keren Kaufman-Francis ; Joshua聽B. Studdert ; Kirsten聽A. Steiner ; Melinda聽D. Power ; David聽A.F. Loebel ; Vanessa Jones ; Angelyn Hor ; Gustavo de聽Alencastro ; Grant聽J. Logan ; Erdahl聽T. Teber ; Oliver聽H. Tam ; Michael聽D. Stutz ; Ian聽E. Alex ; er ; Hilda聽A. Pickett ; Patrick聽P.L. Tam
• 刊名：Cell Stem Cell
• 出版年：2 January, 2014
• 年：2014
• 卷：14
• 期：1
• 页码：107-120
• 全文大小：3805 K

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Summary

Mouse epiblast stem cells (EpiSCs) can be derived from a wide range of developmental stages. To characterize and compare EpiSCs with different origins, we derived a series of EpiSC lines from pregastrula stage to late-bud-stage mouse embryos. We found that the transcriptomes of these cells are hierarchically distinct from those of the embryonic stem cells, induced pluripotent stem cells (iPSCs), and epiblast/ectoderm. The EpiSCs display globally similar gene expression profiles irrespective of the original developmental stage of the source tissue. They are developmentally similar to the ectoderm of the late-gastrula-stage embryo and behave like anterior primitive streak cells when differentiated in聽vitro and in聽vivo. The EpiSC lines that we derived can also be categorized based on a correlation between gene expression signature and predisposition to differentiate into particular germ-layer derivatives. Our findings therefore highlight distinct identifying characteristics of EpiSCs and provide a foundation for further examination of EpiSC properties and potential.