A new bioassay for the ecotoxicological testing of VOCs on groundwater invertebrates and the effects of toluene on Niphargus inopinatus

详细信息    查看全文

• 作者：Maria Avramov ; Susanne I. Schmidt¹ ; Christian Griebler ; ^{christian.griebler@helmholtz-muenchen.de}
• 关键词：Method ; Volatile compound ; Stygobite ; Henry-equilibrium ; Groundwater fauna
• 刊名：Aquatic Toxicology
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：130-131
• 期：Complete
• 页码：1-8
• 全文大小：419 K

文摘

A protocol was developed for testing the ecotoxicological effects of volatile organic compounds (VOCs) on groundwater invertebrates. Test substance volatility was addressed in a ¡°closed from start to analysis¡±-design. Since manifestation of toxic effects may be delayed in ¡®slower metabolizing¡¯ organisms such as groundwater fauna, a time-independent (TI-) approach was adopted. Toluene was used as a model substance and its toxicity to the groundwater amphipod Niphargus inopinatus was assessed as an example. The method evaluation process considered various methodological issues such as partitioning of the toxicant between the water and the gas phase (Henry equilibrium), the possible depletion of oxygen in closed test vials, as well as microbial biodegradation of the test substance. For N. inopinatus, an LC₅₀,_{14 days} of 46.6 mg L^?1 toluene was obtained. The ultimate LC₅₀ value was estimated at 23.3 mg L^?1 toluene. No oxygen depletion occurred in the test vials and Henry equilibrium was found to be established after 6 h. The new test system proposed now awaits broad practical application.