Receptor specificity does not affect replication or virulence of the 2009 pandemic H1N1 influenza virus in mice and ferrets

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• 作者：Seema S. Lakdawala ; Angela R. Shih ; Akila Jayaraman ; Elaine W. Lamir ; e ; Ian Moore ; Myeisha Paskel ; Ram Sasisekharan ; Kanta Subbarao
• 关键词：Influenza virus ; Tissue tropism ; Virulence ; Receptor specificity ; Replication
• 刊名：Virology
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：446
• 期：1-2
• 页码：349-356
• 全文大小：5585 K

文摘

Human influenza viruses predominantly bind ¦Á2,6 linked sialic acid (SA) while avian viruses bind ¦Á2,3 SA-containing complex glycans. Virulence and tissue tropism of influenza viruses have been ascribed to this binding preference. We generated 2009 pandemic H1N1 (pH1N1) viruses with either predominant ¦Á2,3 or ¦Á2,6 SA binding and evaluated these viruses in mice and ferrets. The ¦Á2,3 pH1N1 virus had similar virulence in mice and replicated to similar titers in the respiratory tract of mice and ferrets as the ¦Á2,6 and WT pH1N1 viruses. Immunohistochemical analysis determined that all viruses infected similar cell types in ferret lungs. There is increasing evidence that receptor specificity of influenza viruses is more complex than the binary model of ¦Á2,6 and ¦Á2,3 SA binding and our data suggest that influenza viruses use a wide range of SA moieties to infect host cells.