Analgesic and anti-edematogenic effects of oral trypsin were abolished after subdiaphragmatic vagotomy and spinal monoaminergic inhibition in rats

详细信息    查看全文

• 作者：Flora Lucena ; Vanessa Foletto ; Lucas Zanon Mascarin ; Carlos Rogé ; rio Tonussi ; ^{c.r.tonussi@ufsc.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Enteropeptidase ; Arthritis ; Vagus nerve ; Pain control ; Monoamines ; Inflammation
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 December 2016
• 年：2016
• 卷：166
• 期：Complete
• 页码：60-65
• 全文大小：625 K

文摘

Rheumatoid arthritis brings great burdens to the patients. In addition to the highly expensive treatment, they are commonly associated with severe side effects. In such context, the research for safe and affordable treatments is needed.

Main methods

Arthritis was induced by CFA (0.5 mg/mL) in female wistar rats. Trypsin was given p.o. (2.95 mg/kg; 2 mL) 24 h after the intra-articular CFA injection. Articular incapacitation was measured daily by counting the paw elevation time (PET; s) during 1-min periods of stimulated walk, throughout the 7-days after intra-articular CFA injection. Articular diameter (AD) was accessed just after each PET measurement, taken the difference between naïve and diseased knee-joint diameter (cm).

Key findings

The present study showed that orally administered trypsin was able to reduce nociception and edema, effects that could be observed throughout the evaluation period. These effect, however, were not observed in animals underwent subdiaphragmatic vagotomy, suggesting a vagal mediation for trypsin effects. Likewise, these effects were blocked in rats which received intrathecal injection of the neurotoxins 5,7-dihydroxytryptamine or 6-hydroxydopamine, suggesting the involvement of spinal amines from axon terminals.

Significance

The present study proposes that oral trypsin may cause vagal activation, followed by the activation of descending inhibitory pathways and such mechanism may lead to a novel approach for the treatment of arthritis.