The level and distribution of the GABA_BR2 receptor subunit in the rat's central auditory system

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• 作者：L. Jamal^a ; H. Zhang^a ; P.G. Finlayson^b ; L.A. Porter^a ; H. Zhang^a ; ^{hzhang@uwindsor.ca}
• 关键词：auditory brainstem ; auditory cortex ; cochlear nucleus ; GABA_B receptor ; inferior colliculus ; medial geniculate nucleus
• 刊名：Neuroscience
• 出版年：2011
• 出版时间：5 May 2011
• 年：2011
• 卷：181
• 期：Complete
• 页码：243-256
• 全文大小：7286 K

文摘

The GABA_B receptor is important for the function of auditory neurons. We used Western blotting and immunohistochemical methods to examine the level and localization of GABA_BR2, a required subunit of a functional GABA_B receptor, in the rat's central auditory system. Results revealed that this subunit was expressed throughout the auditory system with the level being high in the layers I–V of the auditory cortex, medial geniculate nucleus, dorsomedial and lateral parts of the inferior colliculus, and the molecular and fusiform cell layers of the dorsal cochlear nucleus. Labeled cell bodies were found in all the areas showing immunoreactivity. Neuropil labeling was strong in areas with high overall levels of immunoreactivity. Regional distributions of the receptor subunit revealed clear boundaries of some auditory subnuclei including the dorsal and ventral cochlear nuclei and the lateral superior olivary nucleus. Differences in immunoreactivity were found between the central nucleus and the dorsal cortex of the inferior colliculus and between the dorsal and ventral parts of the ventral nucleus of the lateral lemniscus, although no clear boundaries were observed. No differences in immunoreactivity were found between the core and the belt areas of the auditory cortex and among the subdivisions of the medial geniculate nucleus. The regional distribution of the receptor subunit in auditory structures is consistent with inputs to these structures and the cellular localization of the receptor in auditory neurons supports the contribution of the GABA_B receptor to synaptic responses in these neurons.