The influences of age on T lymphocyte subsets in C57BL/6 mice

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• 作者：Jing Xie^a ; ^b ; ¹ ; ³ ; ^{isable624@163.com} ; Jin Zhang^c ; ^d ; ² ; ³ ; ^{982632014@qq.com} ; Huimin Wu^e ; Xiaochen Tang^d ; Jie Liu^d ; Guangwen Cheng^c ; ^{chenggw@wust.edu.cn} ; Ping Li^f ; ^{lilyforever76@126.com}
• 关键词：Immunosenescence ; T lymphocyte subsets ; C57BL/6 mice ; Aging
• 刊名：Saudi Journal of Biological Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：24
• 期：1
• 页码：108-113
• 全文大小：969 K
• 卷排序：24

文摘

The aim of this study is to evaluate the age related changes of T lymphocyte subsets in C57BL/6 mice and immune function. Multi-color immunofluorescence techniques that were used to analyse relative numbers of T lymphocyte subsets include CD4+, CD8+, naive and memory CD4+ and CD8+, CD8+CD28+ T cells in peripheral blood of C57BL/6 mice from different age groups (Group I: 2 months old; Group II: 7 months old; Group III: 21 months old); Splenocytes isolated from different group mice were stimulated with Con A to evaluate the proliferative ability. Compared with group I, group II had a significant reduction in the percentage of CD4+, naive CD4+ and CD8+ T cells and an increase in the percentage of CD8+ T cells, while group III had a significant reduction in the percentage of CD4+, naive CD4+ and CD8+ T cells and increase in the percentage of CD8+, memory CD4+ and CD8+ T cells in peripheral blood. Compared with group II, group III had a significant reduction in the percentage of naive CD8+ T cells and increase in the percentage of memory CD4+ and CD8+, CD8+CD28+ T cells in peripheral blood. The T lymphocyte proliferation in vitro showed that groups II and III had a lower proliferative capacity than group I, between groups II and III, there was not a significant difference. We provide relative values for the T lymphocyte subsets in the different age groups of C57BL/6 mice. The immune system began aging at 7 months old in C57BL/6 mice under a specific pathogen free environment.