Yeast rRNA Expansion Segments: Folding and Function
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- 作者：Lizzette M. Gó ; mez Ramos1 ; 2 ; Johanna M. Smeekens1 ; Nicholas A. Kovacs1 ; Jessica C. Bowman1 ; Roger M. Wartell3 ; Ronghu Wu1 ; Loren Dean Williams1 ; loren.williams@chemistry.gatech.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：rRNA ; ribosomal RNA ; ES ; expansion segment ; LSU ; large ribosomal subunit ; ES7 ; expansion segment 7 ; aaRS ; aminoacyl tRNA synthetase ; AspRS ; aspartyl tRNA synthetase ; LysRS ; lysil tRNA synthetase ; TyrRS ; tyrosyl tRNA synthetase ; mRBP ; mRNA-binding protein ; EMSA ; electrophoretic mobility shift assay ; SHAPE ; selective 2&prime ; -hydroxyl acylation analyzed by primer extension ; RT ; reverse transcription
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：9 October 2016
- 年：2016
- 卷：428
- 期：20
- 页码：4048-4059
- 全文大小：1360 K
文摘
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Larger rRNAs of complex organisms confer increased functionality to the ribosome.
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Expansion segment 7 (ES7) binding proteins were characterized by RNA pull-down.
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Isolated ES7 is stable and maintains a near-native secondary and tertiary structure.
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ES7 is a binding hub for proteins essential to expanded ribosomal function.