Identification and partial characterization of Olyra longicaudata (McClelland, 1842) vitellogenins: Seasonal variation in plasma, relative to estradiol-17β and ovarian growth
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- 作者：Pritha Ghosha ; Debabrata Dasa ; Subir K. Juina ; Sudip Hajraa ; Akash Kacharib ; Debangshu N. Dasb ; Panchanan Natha ; Sudipta Maitraa ; sudipta.maitra@visva-bharati.ac.in" class="auth_mail" title="E-mail the corresponding author ; smaitra3@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Vitellogenin ; Estradiol-17β ; ELISA ; Ovary ; Catfish ; Olyra longicaudata
- 刊名：Aquaculture Reports
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：3
- 期：Complete
- 页码：120-130
- 全文大小：2351 K
文摘
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Two forms of VTGs were identified and partially characterized from plasma of E2‒treated male Olyra longicaudata.
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An antibody-capture competitive ELISA has been developed using anti-catfish (Clarias batrachus) VTG antiserum.
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Good parallelism was observed between VTG standard and diluted plasma samples from E2‒treated male and vitellogenic female.
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Increased ovarian weightfromMarch–July correlates well with elevated plasma VTG and E2 levels.