Enhanced production of β-glucosides by in-situ UDP-glucose regeneration
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- 作者：Fong-Chin Huang ; Jens Hinkelmann ; Alexandra Hermenau ; Wilfried Schwab ; schwab@tum.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Glycosyltransferase ; Sucrose synthase ; Glucosylation ; UDP-glucose regeneration
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 April 2016
- 年：2016
- 卷：224
- 期：Complete
- 页码：35-44
- 全文大小：704 K
文摘
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Glucosyltransferases were co-expressed with sucrose synthase (SUS) in E. coli cells.
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SUS enhanced glucosylation efficiency and reduced the amount of required UDP-glucose.
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SUS allows in situ regeneration of UDP-sugars and avoids product inhibition by UDP.