The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex

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• 作者：Haruo Okado ; Chiaki Ohtaka-Maruyama ; Yoshinobu Sugitani ; Yuko Fukuda ; Reiko Ishida ; Shinobu Hirai ; Akiko Miwa ; Akiyo Takahashi ; Katsunori Aoki ; Keiji Mochida ; Osamu Suzuki ; Takao Honda ; Kazunori Nakaj
• 关键词：RP58 ; Transcriptional repressor ; Cerebral cortex ; Apoptosis ; Cell-cycle exit ; Progenitor cell
• 刊名：Developmental Biology
• 出版年：2009
• 出版时间：15 July 2009
• 年：2009
• 卷：331
• 期：2
• 页码：140-151
• 全文大小：5929 K

文摘

The neocortex and the hippocampus comprise several specific layers containing distinct neurons that originate from progenitors at specific development times, under the control of an adequate cell-division patterning mechanism. Although many molecules are known to regulate this cell-division patterning process, its details are not well understood. Here, we show that, in the developing cerebral cortex, the RP58 transcription repressor protein was expressed both in postmitotic glutamatergic projection neurons and in their progenitor cells, but not in GABAergic interneurons. Targeted deletion of the RP58 gene led to dysplasia of the neocortex and of the hippocampus, reduction of the number of mature cortical neurons, and defects of laminar organization, which reflect abnormal neuronal migration within the cortical plate. We demonstrate an impairment of the cell-division patterning during the late embryonic stage and an enhancement of apoptosis of the postmitotic neurons in the RP58-deficient cortex. These results suggest that RP58 controls cell division of progenitor cells and regulates the survival of postmitotic cortical neurons.