Prevalence of Epstein-Barr virus in tonsils and adenoids of United Arab Emirates nationals

详细信息    查看全文

• 作者：Suhail Al-Salam^a ; ^{suhaila@uaeu.ac.ae} ; Shamma Al Dhaheri^b ; Aktham Awwad^b ; Sayel Daoud^b ; Ahmed Shams^b ; Mouied Al Ashari^b
• 关键词：Tonsils ; Adenoids ; EBV ; UAE
• 刊名：International Journal of Pediatric Otorhinolaryngology
• 出版年：2011
• 出版时间：September, 2011
• 年：2011
• 卷：75
• 期：9
• 页码：1160-1166
• 全文大小：1K

文摘

Objective

Given that Epstein-Barr virus (EBV) often inhabits human tonsils and adenoids, it remains to be distinctively determined its prevalence and in which cell and microenvironment the virus is present.

Methods

To determine the prevalence of EBV in the tonsils and adenoids of the United Arab Emirates (UAE) nationals and to provide a basis for understanding the origin and biology of EBV-infected cells, the immunophenotype of all EBV-infected cells in 46 tonsils and 46 adenoids was determined by EBER in situ hybridization and immunohistochemistry with monoclonal antibodies to T cells (CD3), B cells (CD20), and epithelial cells (cytokeratin AE1/AE3), as well as immunostaining with antibodies to EBV latent membrane protein-1 (LMP-1).

Results

EBV was found in 43 % of tonsillectomy specimens and 15 % of adenoidectomy specimens. All EBV-infected cells were found to be B lymphocytes. About 90 % of the infected B cells are found in the interfollicular regions of tonsils and adenoids and the remaining 10 % are found within the follicles. There is no significant association between EBV infection, age (P = 0.324) and gender (P = 0.442).

Conclusion

EBV is associated with tonsillar hypertrophy and is prevalent in 43 % of our cases. EBV is only detected in B lymphocytes and we believe that B lymphocytes are sites of primary infection and latency. In situ hybridization is the gold standard for the detection of EBV in tissue.