Preparation and Evaluation of a C₁₈-Sulfonic Group Dual Modification Chromatographic Stationary Phase

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• 作者：Tao TANG^a ; ^c ; Wei-Bing ZHANG^b ; Ji-Wei XU^a ; Ming-Zhu XIA^a ; Xue-Dong GONG^a ; Feng-Yun WANG^a ; ^{wangfy@njust.edu.cn} ; Tong LI^c
• 关键词：Mixed-mode chromatographic stationary phase ; Reversed phase chromatography ; Hydrophilic interaction chromatography ; 3-Mercaptopropyltrimethoxysilane
• 刊名：Chinese Journal of Analytical Chemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：45
• 期：1
• 页码：56-60
• 全文大小：671 K
• 卷排序：45

文摘

A C18-sulfonic group dual modification chromatographic stationary phase was prepared by one-pot reaction to modify the surface of silica with two modifier of octadecyltrichlorosilane (OTS) and trimethoxysilylpropanethiol (MPS), and then oxidize the thiol group. Under the optimized reaction conditions, the stationary phase with a mole ratio of 3:7 between C18 and sulfonic group was obtained. The morphology and feature of the stationary phase were characterized by scanning electronic microscopy, elemental analysis and infrared spectra. The chromatographic properties of the prepared stationary phase were systematically investigated in different separation modes. Five alkyl benzenes and three nucleosides were separated successfully by reversed phase chromatography and hydrophilic interaction chromatography, respectively. The bovine serum albumin digest was also well separated on the stationary phase. The results indicated that the prepared mixed-mode stationary phase could provide multiple separation mechanism and had potential advantages in separating complex samples and adjusting the selectivity.