Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression
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- 作者：Abhilash R. Jadhava ; Abdul R. Warc ; Ashwini N. Nikama ; Anmol S. Adhava ; Vidya S. Guptab ; Hari C. Sharmac ; Ashok P. Girib ; Vaijayanti A. Tamhanea ; vatamhane@unipune.ac.in" class="auth_mail" title="E-mail the corresponding author ; vatamhane@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：CanPIs ; Capsicum annuum proteinase inhibitors ; BApNA ; N-α-benzoyl-DL-arginine p-nitroanilide ; TPCK ; Na-tosyl-l-phenyl alanine chloromethyl ketone ; TLCK ; Na-p-tosyl-l-lysine chloromethyl ketone ; IRD ; Inhibitor Repeat Domain ; PMSF ; Phenyl methyl sulfonyl fluoride
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：8
- 期：Complete
- 页码：302-309
- 全文大小：827 K
文摘
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CanPI-7 and -22 ingestion led to dose-dependent growth and development retardation in Chilo partellus.
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Ingestion of CanPIs showed up-regulation of proteinase activity and differential proteinase isoforms in C. partellus.
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CanPI-7/-22 induced differential proteinases of C. partellus were sensitive to CanPI-7 and were insensitive to CanPI-22.
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Molecular interaction studies of C. partellus proteinases and CanPIs identified a potent inhibitor.