Construction of an amperometric d-amino acid biosensor based on d-amino acid oxidase/carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine modified gold electrode

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• 作者：Suman Lata ; Bhawna Batra ; Pankaj Kumar ; C.S. Pundir ; ^{pundircs@rediffmail.com}
• 关键词：Amino acid ; Biosensor ; Carbon nanotubes ; Copper nanoparticles ; Enzymes
• 刊名：Analytical Biochemistry
• 出版年：2013
• 出版时间：1 June, 2013
• 年：2013
• 卷：437
• 期：1
• 页码：1-9
• 全文大小：1113 K

文摘

An improved d-amino acid biosensor was constructed based on covalent immobilization of d-amino acid oxidase onto carboxylated mutliwalled carbon nanotube/copper nanoparticles/polyalinine hybrid film electrodeposited on gold electrode. The biosensor exhibited an optimal response within 2 s at pH 8.0 and 30 ¡ãC when polarized at 0.09 V. There was a linear relationship between biosensor response (¦ÌA) and d-alanine concentration ranging from 0.001 to 0.7 mM. The sensitivity of the biosensor was 54.85 ¦ÌA cm^?2 mM^?1 with a lower limit of detection of 0.2 ¦ÌM (signal/noise = 3). The enzyme electrode was used 150 times over a period of 4 months. The biosensor measured the d-amino acid level in fruit juices.