A Potential Structural Switch for Regulating DNA-Binding by TEAD Transcription Factors
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- 作者：Dong-Sun Lee1 ; &dagger ; ; Clemens Vonrhein2 ; &dagger ; ; Diana Albarado3 ; C.S. Raman4 ; Sudha Veeraraghavan4 ; &dagger ; ; sudha.veeraraghavan@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：TEAD ; TEA domain ; TEAD DBD ; TEAD DNA-binding domain ; EMSA ; electrophoretic mobility shift assay ; NTA ; N-terminal arm
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：19 June 2016
- 年：2016
- 卷：428
- 期：12
- 页码：2557-2568
- 全文大小：1283 K
文摘
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The Hippo pathway transcription factor TEAD is found to exist in monomer-multimer equilibrium.
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DNA-binding competent structure of the monomer is preserved in domain-swapped dimer.
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The ∆ L1 TEAD monomer fold is similar to that of the Radialis MYB domain of plants.
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Domain-swapped dimer is unable to bind cooperatively on direct repeat DNA elements.
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The first structure-based mechanism for DNA selectivity of TEAD is identified.