Schistosoma japonicum: Tsunagi/Y14 protein plays a critical role in the development of the reproductive organs and eggs

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• 作者：Cui-Ping Ren ; Peng Zhang ; Wei-Na Zhang ; Da-Ke Huang ; Xue-Mei Jia ; Li Gui ; Miao Liu ; Ji-Jia Shen
• 关键词：Schistosoma japonicum ; Reproduction ; Reproductive organs ; dsRNA ; RNAi ; Tsunagi/Y14
• 刊名：Experimental Parasitology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：135
• 期：2
• 页码：430-436
• 全文大小：2378 K

文摘

Tsunagi/Y14 is an evolutionarily conserved RNA-binding protein that is required for the maintenance of oogenesis and the masculinization of the germ-line in many animal models. We speculated that Tsunagi/Y14 might also regulate reproductive organ development in Schistosoma japonicum (S. japonicum, Sj). Sj Tsunagi/Y14 and control double-stranded RNAs were introduced into schistosomula by electroporation respectively. These transfected schistosomula were cultured in vitro for 1, 3 or 5 days. The mRNA and protein levels of the target gene in the cultured schistosomula were significantly suppressed compared with those of the control group. Furthermore, BALB/c mice were infected with the transfected schistosomula for 6 weeks and were sacrificed to harvest the adult worms. We found that the silencing of Sj Tsunagi/Y14 led to defects in reproductive organs development in both male and female worms. Moreover, it also affected the size, quantity and activity of the eggs in the mice liver. Our findings indicated that Tsunagi/Y14 plays a critical role in the development of reproductive organs and eggs in S. japonicum.