An optical and rapid sandwich immunoassay method for detection of Salmonella pullorum and Salmonella gallinarum based on immune blue silica nanoparticles and magnetic nanoparticles

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• 作者：Qian SunAuthor Vitae ; Guangying ZhaoAuthor Vitae ; Wenchao Dou ; ^{wdou@zjsu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Optical sandwich immunoassay ; Blue silica nanoparticles ; Magnetic nanoparticles ; Salmonella pullorum and Salmonella gallinarum
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：226
• 期：Complete
• 页码：69-75
• 全文大小：1662 K

文摘

An optical and rapid sandwich immunoassay of Salmonella pullorum and Salmonella gallinarum (S. pullorum and S. gallinarum) was designed using anti-S. pullorum and S. gallinarum antibody-functionalized blue silica nanoparticles (IgG-Blue-SiNPs) and magnetic nanoparticles (IgG-MNPs) as immunosensing probes in this article. The IgG-MNPs were used for enrichment of S. pullorum and S. gallinarum, IgG-Blue-SiNPs were used for signal amplification. The Blue-SiNPs were synthesized by doping C.I. reactive blue 14 into silica nanoparticles using an inverse microemulsion method. The morphology, surface charge and functional groups of Blue-SiNps were characterized by SEM, Zeta potential and FTIR spectroscopy. S. pullorum and S. gallinarum in sample solution was captured, enriched and separated by IgG-MNPs. Then IgG-Blue-SiNps were added into the above mixture solution, S. pullorum and S. gallinarum was sandwiched by IgG-MNPs and IgG-Blue-SiNps, forming a blue plaque. Under optimal conditions, the detection limit for pure S. pullorum and S. gallinarum was from 8.8 × 10¹. The detection limit for S. pullorum and S. gallinarum in milk powder was 8.8 × 10² CFU/ml. Besides, this qualitative detection method was economic, simple, rapid, specific and good stability. Such a simple optical sandwich immunoassay holds great potential as an on-site tool for clinical diagnosis of bacteria and viruses.