Green coffee bean extract improves obesity by decreasing body fat in high-fat diet-induced obese mice

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• 作者：Bong-Keun Choi¹ ; ^# ; Sung-Bum Park² ; ^# ; Dong-Ryung Lee¹ ; Hae Jin Lee² ; Ying-Yu Jin³ ; Seung Hwan Yang² ; ³ ; ^{ymichigan@mju.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Joo-Won Suh³ ; ^{jwsuh@mju.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Green coffee bean extract ; High-fat diet induced obese mice ; Adipogenesis ; Body fat ; Dual energy X-ray absorptiometry
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：9
• 期：7
• 页码：635-643
• 全文大小：1553 K

文摘

To evaluate possible lipid catabolism and body fat regulation effects of 3-caffeoylquinic acid in Green coffee bean extract (GCBE) in high-fat diet (HFD)-induced obese mice.

Methods

Obesity was induced in mice using a HFD for four weeks. Then, mice were fed only HFD or HFD with GCBE at 50, 100, and 200 mg/kg. Fatty acid synthesis mechanism regulation of body fat was investigated through real-time PCR and Western blot assay. Body fat reduction was measured through dual-energy X-ray absorptiometry.

Results

In HFD-induced obese mice, GCBE treatment significantly decreased body weight gain, liver weight and white adipose tissue weights with regulation of adipose tissue lipolysis hormones, like adiponectin and leptin. GCBE treatment decreased mRNA expression levels of adipogenesis and adipocyte metabolism related genes in adipose tissues and the liver, and decreased the corresponding protein expression. Dual energy X-ray absorptiometry measurements were used to compare body fat between mice on high-fat and those treated with GCBE. GCBE treated mice had a lower fat mass compared to HFD alone fed mice and relative body weight and fat mass were markedly decreased.

Conclusions

GCBE has a potential anti-obesity effect with lowering body fat accumulation by regulating adipogenesis and lipid metabolism-related genes and proteins in WAT and liver.