3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac repair

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• 作者：Jinah Jang^a ; ¹ ; Hun-Jun Park^b ; ¹ ; Seok-Won Kim^a ; Heejin Kim^b ; Ju Young Park^c ; Soo Jin Na^b ; Hyeon Ji Kim^a ; Moon Nyeo Park^a ; Seung Hyun Choi^d ; Sun Hwa Park^e ; Sung Won Kim^e ; Sang-Mo Kwon^d ; Pum-Joon Kim^b ; ^{bjheart@catholic.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Dong-Woo Cho^a ; ^{dwcho@postech.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：3D printing ; Hydrogel ; Decellularized extracellular matrix ; Stem cells ; Tissue engineering
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：264-274
• 全文大小：3730 K

文摘

Stem cell therapy is a promising therapeutic method for the treatment of ischemic heart diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In this study, stem cell-laden decellularized extracellular matrix bioinks are used in 3D printing of pre-vascularized and functional multi-material structures. The printed structure composed of spatial patterning of dual stem cells improves cell-to-cell interactions and differentiation capability and promotes functionality for tissue regeneration. The developed stem cell patch promoted strong vascularization and tissue matrix formation in vivo. The patterned patch exhibited enhanced cardiac functions, reduced cardiac hypertrophy and fibrosis, increased migration from patch to the infarct area, neo-muscle and capillary formation along with improvements in cardiac functions. Therefore, pre-vascularized stem cell patch provides cardiac niche-like microenvironment, resulting in beneficial effects on cardiac repair.