Identification of proteins regulated by curcumin in cerebral ischemia

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• 作者：Fawad-Ali Shah ; MS^a ; ^b ; Sang-Ah Gim ; DVM ; MS^a ; Jin-Hee Sung ; PhD^a ; Seong-Jun Jeon ; DVM^a ; Myeong-Ok Kim ; PhD^b ; Phil-Ok Koh ; DVM ; PhD^a ; ^{pokoh@gnu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Curcumin ; Stroke ; Proteomics
• 刊名：Journal of Surgical Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：201
• 期：1
• 页码：141-148
• 全文大小：1567 K

文摘

Curcumin is known to have a neuroprotective effect against cerebral ischemia. The objective of this study was to identify various proteins that are differentially expressed by curcumin treatment in focal cerebral ischemia using a proteomic approach.

Methods

Adult male rats were treated with vehicle or curcumin 1 h after middle cerebral artery occlusion. Brain tissues were collected 24 h after the onset of middle cerebral artery occlusion, and cerebral cortices proteins were identified by two-dimensional gel electrophoresis and mass spectrometry.

Results

We detected several proteins with altered expression levels between vehicle- and curcumin-treated animals. Among these proteins, ubiquitin carboxy-terminal hydrolase L1, isocitrate dehydrogenase, adenosylhomocysteinase, and eukaryotic initiation factor 4A were decreased in the vehicle-treated animal, and curcumin treatment attenuated the injury-induced decreases of these proteins. Conversely, pyridoxal phosphate phosphatase was increased in the vehicle-treated animal, and curcumin treatment prevented decreases in this protein. The identified altered proteins are associated with cellular metabolism and differentiation.

Conclusions

The results of this study suggest that curcumin exerts a neuroprotective effect by regulating the expression of various proteins in focal cerebral ischemia.