Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes

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• 作者：Koog Chan Park¹ ; Minkyoung Lee² ; Yoon Jeon³ ; Raok Jeon⁴ ; Sung Hee Baek² ; Ho Lee³ ; Keun Il Kim¹ ; ^{kikim@sookmyung.ac.kr}
• 关键词：CDK ; cyclin-dependent kinase ; E ; embryonic day ; K ; keratin ; P ; postnatal day ; PBS ; phosphate buffered saline
• 刊名：Journal of Investigative Dermatology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：137
• 期：2
• 页码：414-421
• 全文大小：4182 K
• 卷排序：137

文摘

The Mis18 proteins (Mis18α, Mis18β, and M18BP1) are pivotal to the deposition of CENP-A at the centromere during cell cycle progression and are indispensable for embryonic development. Here, we show that Mis18α is critical for the proliferation of keratinocytes and stratification of the epidermis. Mice lacking Mis18α in the epidermis died shortly after birth, showing skin abnormalities like thin and translucent skin and defective skin barrier functions. The epidermis of newborn Mis18α-deficient mice lacked distinct stratification and mature hair follicles, with a reduction in the number of proliferating cells and increased cell death in the basal layer. Earlier expression of the Cre recombinase from keratin-14 promoter in the ventral region resulted in earlier keratinocyte death in the ventral part compared with the dorsal part in the absence of Mis18α, leading to more severe malformation of the ventral epidermal layers. As observed in Mis18α-deficient mouse keratinocytes, knockdown of Mis18α in HaCaT cells caused marked loss of centromeric CENP-A dots and chromosomal misalignment. Overall, we propose that Mis18α is important for epidermal cell proliferation and stratification, because it is required for the deposition of CENP-A at the centromeric nucleosomes.