Anti-NGF monoclonal antibody muMab 911 does not deplete neurons in the superior cervical ganglia of young or old adult rats
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- 作者：John Marceka ; Carlin Okerberga ; Chang-Ning Liua ; David Pottera ; Paul Butlerb ; Magalie Bouchera ; Mark Zorbasb ; Peter Moutonc ; Jens R. Nyengaardd ; Chris Sompsa ; christopher.j.somps@pfizer.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：NGF ; nerve growth factor ; SCG ; superior cervical ganglia ; TH ; tyrosine hydroxylase ; Trk ; tropomyosin receptor kinase ; EGF ; epidermal growth factor ; H&E ; hematoxylin and eosin ; SD ; sprague-dawley ; i.p. ; intraperitoneal ; cv ; cresyl violet ; TB ; toluidine blue ; CE ; coefficient of error ; CV ; coefficient of variance ; AUC ; area under the curve ; HRP ; horseradish peroxidase ; ADA ; antibody drug conjugate ; PK ; pharmacokinetic ; CE ; coefficient of error ; GMA ; glycol methacrylate ; PFA ; paraformaldehyde
- 刊名：Journal of Chemical Neuroanatomy
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：76
- 期：part_PB
- 页码：133-141
- 全文大小：2463 K
文摘
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Neuron counts in the SCG of adult rats were estimated by 2 independent stereology laboratories following anti-NGF treatment.
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Neither young (6–8 weeks) nor fully mature (7–8 months) adult rats lose SCG neurons following anti-NGF treatment.
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Immunohistochemistry minimized detection bias and confirmed no loss of neurons stained with tyrosine hydroxylase.
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These findings are important for understanding the potential risks associated with using anti-NGF therapeutics for treating pain.