The role of insulator elements in defining domains of gene expression

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• 作者：Geyer ; Pamela K
• 关键词：BEAF boundary element associated factor ; HSs hypersensitive sites ; LCRs locus control regions ; MARs matrix attachment regions ; mod(mdg4) modifier of mdg4 ; SARs scaffold attachment regions ; su(Hw) suppressor of Hairy wing
• 刊名：Current Opinion in Genetics & Development
• 出版年：1997
• 出版时间：April, 1997
• 年：1997
• 卷：7
• 期：2
• 页码：242-248
• 全文大小：546 K

文摘

Insulators are naturally occurring DNA sequences that protect transgenes from genomic position effects, thereby establishing independent functional domains within the chromosome. Recent studies have focused on the identification of the cis and trans requirements for insulator activity. These experiments demonstrate that insulators contain multiple components that cooperate to confer their unique properties. Additionally, they suggest that the mechanism of insulation is related to that of enhancer function. Two models of insulator function can be considered: a domain boundary and a transcriptional decoy model.