Suppressor of cytokine signaling 1 expression in peripheral blood mononuclear cells of hepatitis C genotype 1 patients

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• 作者：Chuan-Mo Lee ; Tsung-Hui Hu ; Sheng-Nan Lu ; Jing-Houng Wang ; Chao-Hung Hung ; Chien-Hung Chen ; Yi-Hao Yen ; ^{cassellyen@yahoo.com.tw" class="auth_mail" title="E-mail the corresponding author}
• 关键词：genotype 1 ; hepatitis C virus ; interferon-α ; JAK/STAT signaling ; suppressor of cytokine signaling 1
• 刊名：Journal of the Formosan Medical Association
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：115
• 期：6
• 页码：440-444
• 全文大小：199 K

文摘

While new direct-acting antiviral therapies for hepatitis C virus (HCV) are the standard of care, interferon-α (IFN-α) remains a standard therapy in Taiwan based on its low cost and high response rate to IFN-α-based therapy. IFN-α exerts antiviral activity by inducing the expression of hundreds of genes that establish an antiviral state via Jak kinase (JAK)/signal transducers and activators of transcription (STAT) signaling. We quantified the transcript levels of JAK/STAT signaling genes in peripheral blood mononuclear cells of HCV genotype 1 patients and estimated the correlation between transcript levels and patient responses to IFN-α-based therapy.

Methods

A total of 100 HCV genotype 1 naïve patients were enrolled. All patients received response-guided therapy for 24–48 weeks with pegylated IFN-α and ribavirin. Peripheral blood mononuclear cells were collected before treatment. Twenty patients with sustained virological responses (SVR) and 20 patients without SVR were selected for a JAK/STAT signaling genes transcript analysis using multiplex reverse transcription-polymerase chain reaction. Transcripts that were upregulated or downregulated were further validated in 100 patients.

Results

Suppressor of cytokine signaling 1 (SOCS1) expression was upregulated in SVR patients compared with non-SVR patients (relative quantification = 2.14) based on a multiplex reverse transcription-polymerase chain reaction analysis. We further analyzed SOCS1 expression in 100 patients. We found that SOCS1 expression did not differ significantly between SVR and non-SVR patients.

Conclusion

Peripheral blood SOCS1 expression before treatment was not associated with SVR in HCV genotype 1 patients treated with pegylated IFN-α and ribavirin.