A highly active bile salt hydrolase from Enterococcus faecalis shows positive cooperative kinetics
详细信息 查看全文
- 作者：Deepak Chand ; Sureshkumar Ramasamy ; s.ramasamy@ncl.res.in" class="auth_mail" title="E-mail the corresponding author ; C.G. Suresh ; cg.suresh@ncl.res.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bile salt hydrolase ; Gut organism ; Bile acids ; Allosteric regulation ; Penicillin V acylase ; Enzyme kinetics
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：51
- 期：2
- 页码：263-269
- 全文大小：1541 K
文摘
- •
The cloning, expression and purification of BSH from Enterococcus faecalis were elucidated.
- •
Biochemical and biophysical studies of EfBSH were conducted.
- •
The enzyme showed high activity and positive cooperativity.
- •
Pen V exerted a modulating effect on BSH activity towards conjugated bile acids.