Biochemical and molecular analysis of Camellia sinensis (L.) O. Kuntze tea from the selected P/11/15 clone

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• 作者：Ramkumar Samynathan^a ; ^{ramkumarbiochem007@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Suresh kumar Perisamy^b ; Sudhakar Gandhi^b ; J. Anitha^a ; Geetha Sanmugam^c ; Mohankumar Padmanabhan^d ; Gopalakrishnan Velliyur Kanniappan^a
• 关键词：Camellia sinensis (L) O. Kuntze ; P/11/15 clone ; Biochemical characterization ; Gene expression analysis
• 刊名：Journal of Genetic Engineering and Biotechnology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：14
• 期：1
• 页码：69-75
• 全文大小：800 K

文摘

Green tea is one of the most important beverages consumed across the world and it possesses various phytotherapeutics. Polyphenol oxidase (PPO) activity, total polyphenols, catechins, amino acid content and enzymatic antioxidants are considered to be potential parameters in tea characterization. P/11/15 clone (Camellia sinensis (L) O. Kuntze) was chosen to analyze the biochemical characterization and to analyze the gene expression pattern. The selected P/11/15 clone (C. sinensis (L) O. Kuntze) possess potent Polyphenol oxidase (49.62 U/mg of protein), sufficient catechin (20.75%), Polyphenol (20.01%), Peroxidase (450.08 μM of O₂ formed min⁻¹ g⁻¹ dry weight), Catalase (1.20 μM H₂O₂ reduced min⁻¹ mg⁻¹ protein) and Super Oxide Dismutase (45.11 U/mg proteins). Flavonoid gene expression reveals ANR (1.66%) and F3H (1.02%) were up regulated in the selected P/11/15 clone. The results obtained suggest that P/11/15 clone showed adequate enzyme levels, thus an increased antioxidant activity.