Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1)

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• 作者：Natsumi Takeyama^a ; ^{n-takeyama@nibs.or.jp} ; ^{mis-take@pg7.so-net.ne.jp} ; Kenji Minari^b ; Masahiro Kajihara^b ; Norikazu Isoda^b ; Ryuichi Sakamoto^c ; Takashi Sasaki^d ; Norihide Kokumai^d ; Noriyasu Takikawa^e ; Rikiya Shiraishi^f ; Masaji Mase^g ; Junko Hagiwara^a ; Toshiaki Kodama^a ; Takashi Imamura^c ; Masashi Sakaguchi^c ; Toshiaki Ohgitani^d ; Akira Sawata^e ; Masatoshi Okamatsu^g ; Masatake Muramatsu^f ; Kenji Tsukamoto^g ; Zhifeng Lin^a ; Kotaro Tuchiya^a ; Yoshihiro Sakoda^b ; Hiroshi Kida^b ; ^h
• 关键词：Avian influenza ; NS1-ELISA ; DIVA system
• 刊名：Veterinary Microbiology
• 出版年：2011
• 出版时间：27 January 2011
• 年：2011
• 卷：147
• 期：3-4
• 页码：283-291
• 全文大小：300 K

文摘

H5 and H7 highly pathogenic avian influenza virus (HPAIV) represent a major global concern in poultries and human health. Avian influenza (AI) vaccines are available but not preferred for field applications, primarily because vaccination interferes with sero-surveillances of AIV infection. To overcome the problem, ELISA systems using non-structural protein 1 (NS1) of AIV as antigens (NS1-ELISA) have been developed to measure anti-NS1 antibodies that are raised in AIV-infected but not in vaccinated chickens. However, some AI-vaccinated chickens having a weak anti-virus immune response may subsequently be infected with AIV and spread the virus. This raises a concern for the validity of NS1-ELISA to detect AIV infection in previously vaccinated chickens. In this study, we developed NS1-ELISA and assessed its feasibility to detect HPAIV infection in chickens previously immunized with H5 or H7 AI vaccines. The results indicated that the NS1-ELISA could identify HPAIV infection in both unvaccinated and vaccinated chickens at 1 week after infection in correlation with results from time-consuming virus isolation tests. Taken together, the NS1-ELISA system would be valuable tool to define HPAIV infection when AI vaccine program is in place.