Effects of subtoxic concentrations of TiO₂ and ZnO nanoparticles on human lymphocytes, dendritic cells and exosome production

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• 作者：Britta Andersson-Willman ; Ulf Gehrmann ; Zekiye Cansu ; Tina Buerki-Thurnherr ; Harald F. Krug ; Susanne Gabrielsson ; Annika Scheynius
• 关键词：Nanoparticles ; Human peripheral blood cells ; Dendritic cells ; Natural killer cells ; Exosomes
• 刊名：Toxicology and Applied Pharmacology
• 出版年：2012
• 出版时间：1 October, 2012
• 年：2012
• 卷：264
• 期：1
• 页码：94-103
• 全文大小：1633 K

文摘

Metal oxide nanoparticles are widely used in the paint and coating industry as well as in cosmetics, but the knowledge of their possible interactions with the immune system is very limited. Our aims were to investigate if commercially available TiO₂ and ZnO nanoparticles may affect different human immune cells and their production of exosomes, nano-sized vesicles that have a role in cell to cell communication. We found that the TiO₂ or ZnO nanoparticles at concentrations from 1 to 100 ¦Ìg/mL did not affect the viability of primary human peripheral blood mononuclear cells (PBMC). In contrast, monocyte-derived dendritic cells (MDDC) reacted with a dose dependent increase in cell death and caspase activity to ZnO but not to TiO₂ nanoparticles. Non-toxic exposure, 10 ¦Ìg/mL, to TiO₂ and ZnO nanoparticles did not significantly alter the phenotype of MDDC. Interestingly, ZnO but not TiO₂ nanoparticles induced a down regulation of Fc¦ÃRIII (CD16) expression on NK-cells in the PBMC population, suggesting that subtoxic concentrations of ZnO nanoparticles might have an effect on Fc¦ÃR-mediated immune responses. The phenotype and size of exosomes produced by PBMC or MDDC exposed to the nanoparticles were similar to that of exosomes harvested from control cultures. TiO₂ or ZnO nanoparticles could not be detected within or associated to exosomes as analyzed with TEM. We conclude that TiO₂ and ZnO nanoparticles differently affect immune cells and that evaluations of nanoparticles should be performed even at subtoxic concentrations on different primary human immune cells when investigating potential effects on immune functions.