Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters
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- 作者：Sergej Wendlera ; Andreas Ottob ; Vera Ortseifena ; c ; Florian Bonnb ; Armin Neshatc ; Susanne Schneiker-Bekela ; Timo Wolfa ; c ; Till Zemked ; Udo F. Wehmeiere ; Michael Heckerb ; Jö ; rn Kalinowskic ; Dö ; rte Becherb ; Alfred Pü ; hlera
- 关键词：Actinoplanes ; Comprehensive proteomics ; Metabolic labeling ; Subcellular fractionation ; Acarbose
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：131
- 期：Complete
- 页码：140-148
- 全文大小：1263 K
文摘
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Global comparative study applying subcellular fractionation and metabolic labeling
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Conditions for production of acarbose or acarviosyl-glucose were analyzed.
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Relevant differences are not in acarbose gene cluster, but in saccharide transporters.
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Proteins for transport of maltose (AglEFG & MalEFG) and glucose (MstEAF) proposed.