Comparison of functional limbal epithelial stem cell isolation methods
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- 作者：Marina Ló ; pez-Paniaguaa ; b ; 3 ; marina@ioba.med.uva.es" class="auth_mail" title="E-mail the corresponding author ; Teresa Nieto-Miguelb ; a ; tnietom@ioba.med.uva.es" class="auth_mail" title="E-mail the corresponding author ; Ana de la Mataa ; b ; 1 ; anus1981@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Marc Dziaskoc ; marc.dziasko.11@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Sara Galindoa ; b ; sgalindor@ioba.med.uva.es" class="auth_mail" title="E-mail the corresponding author ; Esther Reyb ; a ; esther_fdz_rey@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; José ; M. Herrerasa ; b ; herreras@ioba.med.uva.es" class="auth_mail" title="E-mail the corresponding author ; Rosa M. Corralesb ; a ; 2 ; rcorrales@pointguardllc.com" class="auth_mail" title="E-mail the corresponding author ; Julie T. Danielsc ; j.daniels@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Margarita Calongea ; b ; 3 ; calonge@ioba.med.uva.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cell culture ; Ocular surface ; Limbal stem cells ; Explants ; Cell suspensions
- 刊名：Experimental Eye Research
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：146
- 期：Complete
- 页码：83-94
- 全文大小：2551 K
文摘
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Optimal method to isolate limbal epithelial stem cells (LESCs) is not established yet.
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Suspension culture LESC protein expression was similar or higher than explant culture.
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Cells cultured from cell suspensions generated more new colonies and meroclones.
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Cultures from cell suspensions had a cell population richer in functional LESCs.
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We compared explant and cell suspension methods for LESC cultivation on plastic.