O-glycans and O-glycosylation sites of recombinant human GM-CSF derived from suspension-cultured rice cells, and their structural role
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- 作者：Jihye Kima ; 1 ; Heajin Parka ; 1 ; Byung Tae Parka ; Hye Seong Hwanga ; Jae Il Kimb ; Dae Kyong Kimc ; Ha Hyung Kima ; hahyung@cau.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Human GM-CSF ; Rice cell ; O-glycosylation ; Structural stability ; In vivo half-life
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：14 October 2016
- 年：2016
- 卷：479
- 期：2
- 页码：266-271
- 全文大小：768 K
文摘
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O-glycosylation of rice-cell-derived recombinant human GM-CSF was investigated.
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Eight O-glycans comprising four Ara with zero to seven Gal were determined.
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O-glycans are heterogeneously attached to Ser 5, Ser 7, Ser 9, or Thr 10 residues.
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Glycans on rrhGM-CSF are essential to the stability of its α-helix structure.
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This is the first report on O-glycosylation of human GM-CSF derived from plant cells.