- 作者：Satoshi Hirayama ; Saori Nakagawa ; Satoshi Soda ; Yumiko Kamimura ; Emiko Nishioka ; Tsuyoshi Ueno ; Yoshifumi Fukushima ; Ken-ichi Higuchi ; Masanori Inoue ; Utako Seino ; Hirotoshi Ohmura ; Susumu Yamato ; Takashi Miida
- 关键词：Plant sterols ; Cholesterol precursors ; Oxysterols ; Ezetimibe ; NPC1L1 ; Gas chromatography&ndash ; mass spectrometry (GC&ndash ; MS)
- 刊名：Atherosclerosis
- 出版年：2013
- 出版时间：September, 2013
- 年：2013
- 卷：230
- 期：1
- 页码：48-51
- 全文大小：644 K
Objective
Cholesterol and diet-derived oxidized cholesterol are absorbed in the small intestine and eliminated by bile acids. We determined whether ezetimibe, a selective cholesterol absorption inhibitor, changes serum oxidized cholesterol levels.Methods
We measured levels of plant sterols, cholesterol precursors, and oxysterols by gas chromatography-mass spectrometry in 47 hypercholesterolemics and 32 controls. Twenty-four hypercholesterolemics received 10?mg ezetimibe/day for 4 weeks.
Results
Plant sterols were 30-42 % higher in hypercholesterolemics than in controls and positively correlated with low-density lipoprotein-cholesterol (LDL-C). Ezetimibe decreased plant sterols by 21-53 % , but did not change bile acid synthesis markers. 7¦Â-hydroxycholesterol, a marker for non-enzymatic oxidation of cholesterol, was 66 % higher in hypercholesterolemics than controls. Ezetimibe decreased 7¦Â-hydroxycholesterol levels by 15 % regardless of LDL-C reduction.
Conclusions
Ezetimibe decreases serum oxidized cholesterol generated by non-enzymatic reactions without impairing bile acid synthesis. Ezetimibe may maintain cholesterol excretion into bile and alleviate the diet-derived oxidative burden.