Structure-function relationship in the CYP74 family: Conversion of divinyl ether synthases into allene oxide synthases by site-directed mutagenesis
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- 作者：Yana Y. Toporkova ; Valeria S. Ermilova ; Svetlana S. Gorina ; Lucia S. Mukhtarova ; Elena V. Osipova ; Yuri V. Gogolev ; Alex ; er N. Grechkin
- 关键词：DES ; divinyl ether synthase ; NtDES ; Nicotiana tabacum DES (CYP74D3) ; LuDES ; Linum usitatissimum DES (CYP74B16) ; AOS ; allene oxide synthase ; HPL ; hydroperoxide lyase ; WT ; wild type ; IHCD ; I-helix central domain ; SRS ; substrate recognition site ; 9-HPOD ; (9S
- 刊名：FEBS Letters
- 出版年：2013
- 出版时间：19 August, 2013
- 年：2013
- 卷：587
- 期：16
- 页码：2552-2558
- 全文大小：1819 K
文摘
Non-classical P450s of CYP74 family control several enzymatic conversions of fatty acid hydroperoxides to bioactive oxylipins in plants, some invertebrates and bacteria. The family includes two dehydrases, namely allene oxide synthase (AOS) and divinyl ether synthase (DES), and two isomerases, hydroperoxide lyase (HPL) and epoxyalcohol synthase. To study the interconversion of different CYP74 enzymes, we prepared the mutant forms V379F and E292G of tobacco (CYP74D3) and flax (CYP74B16) divinyl ether synthases (DESs), respectively. In contrast to the wild type (WT) enzymes, both mutant forms lacked DES activity. Instead, they produced the typical AOS products, ¦Á-ketols and (in the case of the flax DES mutant) 12-oxo-10,15-phytodienoic acid. This is the first demonstration of DES into AOS conversions caused by single point mutations.