Single-dose, virus-vectored vaccine protection against Yersinia pestis

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• 作者：Anasuya Chattopadhyay ; Steven Park ; Guillaume Delmas ; Rema Suresh ; Svetlana Senina ; David S. Perlin ; John K. Rose
• 关键词：Yersinia pestis ; Vesicular stomatitis virus ; CD4⁺ cells
• 刊名：Vaccine
• 出版年：2008
• 出版时间：25 November 2008
• 年：2008
• 卷：26
• 期：50
• 页码：6329-6337
• 全文大小：963 K

文摘

We have developed an experimental recombinant vesicular stomatitis virus (VSV) vectored plague vaccine expressing a secreted form of Yersinia pestis low calcium response protein V (LcrV) from the first position of the VSV genome. This vector, given intramuscularly in a single dose, induced high-level antibody titers to LcrV and gave 90–100 % protection against pneumonic plague challenge in mice. This single-dose protection was significantly better than that generated by VSV expressing the non-secreted LcrV protein. Increased protection correlated with increased anti-LcrV antibody and a bias toward IgG2a and away from IgG1 isotypes. We also found that the depletion of CD4⁺ cells, but not CD8⁺ cells, at the time of challenge resulted in reduced vaccine protection, indicating a role for cellular immunity in protection.