Fullerene C60 films of continuous and micropatterned morphology as substrates for adhesion and growth of bone cells
详细信息 查看全文
- 作者：Lubica Grausova ; Jiri Vacik ; Vladimir Vorlicek ; Vaclav Svorcik ; Petr Slepicka ; Petra Bilkova ; Marta V ; rovcova ; Vera Lisa ; Lucie Bacakova
- 关键词：Nanoparticles ; Biocompatibility ; Surface structure ; Biomedical application
- 刊名：Diamond and Related Materials
- 出版年：2009
- 出版时间：February-March 2009
- 年：2009
- 卷：18
- 期：2-3
- 页码：578-586
- 全文大小：2297 K
文摘
Fullerenes C60 were deposited on microscopic glass coverslips in the form of continuous layers (thickness 505 ± 43 nm or 1090 ± 8 nm) or micropatterned layers using a metallic mask with rectangular openings (128 × 98 µm, spacing 50 µm). Below the openings, the fullerenes formed bulges 484 ± 5 nm or 1043 ± 57 nm in thickness. Very thin fullerene films were also found below the metallic bars of the grid. The adhesion and proliferation activities of human osteoblast-like MG 63 cells in cultures on the fullerene layers were similar as that on the control polystyrene dishes and glass coverslips. On the thick patterned layers, the cells grew preferentially in the grooves among the fullerene bulges. Although these grooves occupied only approximately 41 % of the surface, they contained from 80 % to 98 % of the cells. Immunocytochemistry showed that the cells on all tested surfaces formed β1 integrin- and talin-containing focal adhesion plaques, a β-actin cytoskeleton, and contained osteocalcin, a marker of osteogenic cell differentiation. These results suggest that fullerene C60 layers can act as good substrates for cell colonization, and could also serve for the construction of micropatterned surfaces for guided cell adhesion and growth.