High-level production of (5S)-hydroxyhexane-2-one by two thermostable oxidoreductases in a whole-cell catalytic approach
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- 作者：Sylvia Diederichsa ; sylvia.diederichs@avt.rwth-aachen.de" class="auth_mail" title="E-mail the corresponding author ; Katharina Linna ; katharina.linn@rwth-aachen.de" class="auth_mail" title="E-mail the corresponding author ; Janine Lü ; ckgena ; janine.lueckgen@rwth-aachen.de" class="auth_mail" title="E-mail the corresponding author ; Tobias Klementa ; tobias.klement@avt.rwth-aachen.de" class="auth_mail" title="E-mail the corresponding author ; Jan-Hendrik Groschb ; janhendrik.grosch@avt.rwth-aachen.de" class="auth_mail" title="E-mail the corresponding author ; Kohsuke Hondac ; honda@bio.eng.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Hisao Ohtakec ; hohtake@bio.eng.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Jochen Bü ; chsa ; jochen.buechs@avt.rwth-aachen.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：BIS-TRIS ; bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane ; CDW ; cell dry weight ; CHES ; 2-(cyclohexylamino)ethanesulfonic acid ; E. coli ; Escherichia coli ; HBD ; 3-hydroxybutyryl-CoA dehydrogenase ; GC ; gas chromatography ; GDH ; glucose 1-dehydrogenase ; HPLC ; high-performance liquid chromatography ; MES ; 2-(N-morpholino)ethanesulfonic acid ; MOPS ; 3-(N-morpholino)propanesulfonic acid ; OD ; optical density ; OnEx medium ; overnight Express auto-induction medium ; OTR ; oxygen transfer rate ; RAMOS ; resp
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：121
- 期：Complete
- 页码：37-44
- 全文大小：1128 K
文摘
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Production of (5S)-hydroxyhexane-2-one with thermostable enzymes investigated.
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HBD identified as S-selective enzyme withstanding high substrate concentrations.
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Production of optically pure (5S)-hydroxyhexane-2-one at a final concentration of 169 ± 3 mM.
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Thermostable whole-cell catalyst superior to published catalysts.