Outer membrane vesicles (OMV) production of Neisseria meningitidis serogroup B in batch process

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• 作者：S¨ªlvia Santos ; Luciana Juncioni de Arauz ; J¨²lia Baruque-Ramos ; Ivo Lebrun ; Sylvia Mendes Carneiro ; S ; ra Alves Barreto ; Rocilda Perazzini Furtado Schenkman
• 关键词：Neisseria meningitidis ; Outer membrane vesicles ; Vaccine ; Bioreactor systems ; Kinetics
• 刊名：Vaccine
• 出版年：2012
• 出版时间：14 September, 2012
• 年：2012
• 卷：30
• 期：42
• 页码：6064-6069
• 全文大小：535 K

文摘

Serogroup B outer membrane vesicles (OMV) with iron regulated proteins (IRP) from Neisseria meningitidis constitute the antigen for the vaccine against the disease caused by this bacterium. Aiming to enhance final OMV concentration, seven batch experiments were carried out under four different conditions: (i) with original Catlin medium; (ii) with original Catlin medium and lactate and amino acids pulse at the 6th cultivation hour; (iii) with Catlin medium with double initial concentrations of lactate and amino acids and (iv) Catlin medium without glycerol and with double initial concentrations of lactate and amino acids. The cultivation experiments were carried out in a 7-L bioreactor under the following conditions: 36 ¡ãC, 0.5 atm, overlay air 1 L/min, agitation: 250-850 rpm, and O₂ control at 10 % , 20 h. After lactate and amino acids exhaustion, cell growth reached stationary phase and a significant release increase of OMV was observed. According to the Luedeking & Piret model, OMV liberation is non-growth associated. Glycerol was not consumed during cultivation. The maximum OMV concentration value attained was 162 mg/L with correspondent productivity of 8.1 mg/(L h) employing Catlin medium with double initial concentrations of lactate and amino acids. The obtained OMV satisfied constitution and protein pattern criteria and were suitable for vaccine production.