Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology

详细信息    查看全文

• 作者：Catherine Bonnefont-Rebeix^a ; ^{catherine.bonnefont@vetagro-sup.fr" class="auth_mail" title="E-mail the corresponding author} ; Corinne Fournel-Fleury^a ; Fré ; dé ; rique Ponce^a ; Sara Belluco^a ; Dorothé ; e Watrelot^a ; Sylvie E Bouteille^a ; Sylvie Rapiteau^a ; Diane Razanajaona-Doll^b ; Jean-Jacques Pin^b ; Caroline Leroux^c ; Thierry Marchal^a
• 关键词：T-cell lymphoma ; Cell line ; Dog ; IL-17 ; CD56 mRNA
• 刊名：Immunobiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：221
• 期：1
• 页码：12-22
• 全文大小：1865 K

文摘

Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNγ and TNFα mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRγ gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5 h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.