Antimicrobial efficiency of carvacrol vapour related to mass partition coefficient when incorporated in chitosan based films aimed for active packaging

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• 作者：Mia Kurek^a ; ^b ; Sylvie Moundanga^a ; Coralie Favier^c ; Kata Gali?^b ; Fré ; dé ; ric Debeaufort^a ; ^c ; ^{frederic.debeaufort@u-bourgogne.fr}
• 关键词：Chitosan ; Carvacrol ; Headspace ; Antimicrobial ; Mass partition coefficient ; Bacteria
• 刊名：Food Control
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：32
• 期：1
• 页码：168-175
• 全文大小：1498 K

文摘

Chitosan-based packaging films were investigated for antimicrobial efficiency against food spoilage microorganisms through headspace technique. Five chitosan based films containing carvacrol with known mass partition coefficient (K_mass) in the range from 10^?4 to 10^?8 were produced and their antimicrobial activity was evaluated. Bacillus subtilis, Escherichia coli, Listeria innocua and Salmonella enteritidis were exposed to active films either just after inoculation or just after an incubation phase. When tests were made at inoculation time for carvacrol concentration of 3.13?¡Á?10^?8?g?mL^?1 and low K_mass, films revealed antimicrobial efficiency against all tested microorganisms. When already grown colonies were exposed to films, those with lower K_mass displayed moderate inhibitory action against tested Gram-negative and strong efficiency against tested Gram-positive bacteria. From all results, the minimal vapour inhibitory concentration was considered as 4.62?¡Á?10^?8?g?mL^?1 (K_mass?=?1.13?¡Á?10^?6) for B.?subtilis, E.?coli and L.?innocua, 1.08?¡Á?10^?7?g?mL^?1 (K_mass?=?1.01?¡Á?10^?4) for S. enteritidis. Films with the carvacrol vapour concentrations of 4.62?¡Á?10^?8?g?mL^?1 and 6.41?¡Á?10^?8?g?mL^?1 had strong antimicrobial effect at t₀ and t₁ except for S. enteritidis that was the most resistant bacterium. Concentration of 3.13?¡Á?10^?8?g?mL^?1 was efficient at t₀ but not at t₁. Concentration of 6.28?¡Á?10^?9?g?mL^?1 did not have any antimicrobial effect against all tested microorganisms, both at t₀ and t₁. Lag phase, doubling time of bacteria and concentration of released volatile compounds are key factors of antimicrobial efficacy. Antimicrobial tests and observations permitted a comparison of bioactivity potential of the activated chitosan films and the examination of the relationship between the antimicrobial properties and the mass partition coefficient.