文摘
A new porous silicon dioxide chip (DIOSD) for matrix-free laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF MS) has been presented. Spots of porous silicon dioxide were fabricated by electrochemical etching of a silicon substrate followed by the high temperature wet oxidation. It was shown, that the porous silicon dioxide ensures effective laser-induced desorption and ionisation of biomolecules. The noise background signal of the DIOSD chip was low, and did not interfere to the measurements. The 50fmol detectability of tripeptide LGG was obtained. Spectrograms of the selected catecholamines (dopamine, norepinephrine) and captopril in the range of a few dozen picomoles were successfully done. Is has been shown, that the DIOSD chip may successfully replace the standard matrix-assisted target plate, used in the proteomics/peptideomics mass spectrometry, especially for low-mass biomolecules analysis.