文摘
The new fiber-based optical microfluidic detectors, designed for nano-volume sample measurement, are presented. The silicon-glass microdetectors were fabricated by use of micromechanical techniques. Spectrofluorimetric and spectrophotometric methods of detection were applied. Optical fibers, integrated into microchannels, ensured very low dead-volume (17nl for spectrofluorimetric and 900nl for spectrophotometric detector). The microdevices were made as separate modules and may be connected to any microanalytical system, in which the fluorescence or absorbance is measured. The fabrication methods of microdetectors are compatible with micro-total analysis system (μTAS) technology. The preliminary tests showed the linear detection range 0–0.2mg/ml of fluorescein and erythrosine (spectrofluorimetric detector), and 0.2–1.6μg P/ml of phosphate in water solutions (spectrophotometric detector).