Synergetic stress of acids and ammonium on the shift in the methanogenic pathways during thermophilic anaerobic digestion of organics
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文摘
Combined effects of acids and ammonium on functional pathway and microbial structure during organics methanization were investigated by stable isotopic method and quantitative PCR. The results showed that the stress from acids and ammonium was synergetic, resulted in different inhibition for acetoclastic and hydrogenotrophic methanogenesis and syntrophic acetate oxidation, leading to pathway shift. Methane production from acetate was affected more by acetate than by ammonium until the ammonium concentration reached 6-7?g-N/L. When the ammonium concentration exceeded 6?g-N/L, ammonium inhibition was strengthened by the increased concentration of acetate. At a low acetate concentration (50?mmol/L), acetoclastic methanogenesis dominated, regardless of ammonium concentration. At higher acetate concentrations (150 and 250?mmol/L) and at low-medium ammonium levels (1-4?g-N/L), acetate was mainly degraded by acetoclastic methanogenesis, while residual acetate was degraded by a combination of acetoclastic methanogenesis and the syntrophic reaction of syntrophic acetate oxidization and hydrogenotrophic methanogenesis with the latter dominating at 250?mmol/L acetate. At high ammonium levels (6-7?g-N/L), the degradation of acetate in the 150?mmol/L treatment was firstly through a combination of acetoclastic methanogenesis and the syntrophic pathway and then gradually shifted to the syntrophic pathway, while the degradation of acetate in the 250?mmol/L treatment was completely by the syntrophic pathway.

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