Neurotrophin serum concentrations and polymorphisms of neurotrophins and their receptors in children with asthma

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• 作者：Aleksandra Szczepankiewicz^a ; ^b ; ^{alszczep@amp.edu.pl} ; Marta Rachel^c ; Paulina Sobkowiak^b ; Zdzisł ; awa Kycler^b ; Irena Wojsyk-Banaszak^b ; Natalia Schö ; neich^b ; Aleksandra Szczawiń ; ska-Popł ; onyk^b ; Anna Brę ; borowicz^b
• 关键词：Asthma ; Neurotrophin ; Gene ; Polymorphism ; Serum level
• 刊名：Respiratory Medicine
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：107
• 期：1
• 页码：30-36
• 全文大小：338 K

文摘

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Summary

Background

In the recent years numerous studies have analysed the effects of neurotrophins on allergic inflammation in airway diseases reporting increased neurotrophin levels locally in the airways as well as in serum of asthmatic patients. We aimed to investigate if levels of neurotrophins in serum of asthmatic children are influenced by the genotype of functional variants within genes encoding analysed neurotrophins and their specific receptors.

Methods

In the study we included 98 children diagnosed with asthma. Genotyping of 9 polymorphisms located in neurotrophins genes and their receptors genes was done with use of TaqMan SNP genotyping assays or PCR-RFLP. The serum levels of four neurotrophins (BDNF, NGF, NTF3, NTF4) were analysed during exacerbation of asthma symptoms with use of DuoSet ELISA Development Kit (R&D).

Results

The two patients with the genetic variant A/A of NTRK1 (rs6334) showed significantly higher NGF serum concentrations (113.4 and 218.1?pg/mL) as compared to the mean NGF serum concentrations in the total group of patients (34.8?pg/mL). We also observed a significant epistatic interactions between variants of NGF rs6330 and NTRK1 rs6334 that influenced NGF serum level (P?=?0.0004). Analysis of four neurotrophins serum levels in relation to different genotypes of analysed neurotrophins genes showed no significant differences among analysed asthmatic children.

Conclusions

Our results suggest that, among analysed neurotrophins, NGF serum levels may be influenced by the genotype of NTRK1 gene individually as well as in the interaction with NGF functional genetic variant suggesting their involvement in allergic inflammation in asthma. Serum levels of the other neurotrophins do not seem to be affected by the variants in the analysed genes.