Factor XIII subunits in human tears; their highly elevated levels following penetrating keratoplasty

详细信息    查看全文

• 作者：Zsuzsanna Z. Orosz ; É ; va Katona ; Andrea Facsk&#xf3 ; Lá ; szl&#xf3 ; M&#xf3 ; dis ; Lá ; szl&#xf3 ; Muszbek ; Andrá ; s Berta
• 关键词：Factor XIII ; Tears ; Corneal transplantation ; Penetrating keratoplasty ; Neovascularization ; Wound healing
• 刊名：Clinica Chimica Acta
• 出版年：2011
• 出版时间：30 January 2011
• 年：2011
• 卷：412
• 期：3-4
• 页码：271-276
• 全文大小：503 K

文摘

Background

As blood coagulation factor XIII (FXIII) is of high importance in wound healing, we determined the concentrations of FXIII A and B subunits (FXIII-A and FXIII-B) and their complex (FXIII-A₂B₂) in normal tears and in tears from patients undergoing penetrating keratoplasty (PKP).

Methods

FXIII complex and subunit concentrations were measured by highly sensitive chemiluminescent ELISAs in tears from 60 healthy volunteers and from 31 patients undergoing corneal transplantation.

Results

In non-stimulated tears from healthy volunteers, low but consistent amounts of FXIII-A and FXIII-B (medians: 2.13 μg/L and 7.22 μg/L, respectively) were measured, mostly in non-complexed form. Following stimulation of tear secretion FXIII levels moderately decreased, but if normalized to protein concentration they did not change. One day after PKP FXIII levels became highly elevated, then gradually decreased, but even on day 7 significantly exceeded pre-surgery values. The elevation of tear FXIII levels was significantly higher in PKP patients who later developed neovascularization of donor cornea.

Conclusions

FXIII subunits are low concentration components of normal tear. The striking elevation of FXIII subunit and FXIII-A₂B₂ concentrations after PKP suggests the involvement of FXIII in corneal wound healing. Perioperatively measured high FXIII levels in tears seem to represent a risk of neovascularization.