Fluorous-assisted metal chelate affinity extraction technique for analysis of protein kinase activity
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- 作者：Tadashi Hayama ; Ena Kiyokawa ; Hideyuki Yoshida ; Osamu Imakyure ; Masatoshi Yamaguchi ; Hitoshi Nohta ; nohta@fukuoka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Fluorous ; Metal chelate affinity ; Protein kinase ; Phosphopeptide ; Fluorometry measurement
- 刊名：Talanta
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：156-157
- 期：Complete
- 页码：1-5
- 全文大小：702 K
文摘
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A novel fluorous biphasic extraction method was applied to analysis of protein kinase activity.
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Fe(III)-immobilized PFIDA was utilized for extraction of phosphopeptides to fluorous phase.
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Fluorescent substrate peptide remained in aqueous phase was easily measured.
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This method was successfully applied to assay PKA activity.