Cloning and nucleotide sequence of the major capsid proteins of Lactobacillus bacteriophage φgle

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• 作者：Kakikawa ; Makiko ; Oki ; Masaya ; Tadokoro ; Hisayuki ; Nakamura ; Shogo ; Taketo ; Akira ; Kodaira ; Ken-Ichi
• 关键词：Temperate phage ; Lactobacillus ; Capsid protein ; Phage structural gene ; Protein sequence ; DNA sequence
• 刊名：Gene
• 出版年：1996
• 出版时间：October 10, 1996
• 年：1996
• 卷：175
• 期：1-2
• 页码：157-165
• 全文大小：1.03 M

文摘

Bacteriophage φgle was induced from a lysogenic Lactobacillus strain Gle. φgle genome is double-strained DNA of approximately 42.5 kilo-base (kb) pairs. SDS poly-acrylamide gel electrophoresis demonstrated that the phage particles contain 4 major structural (capsid) proteins, gpB, gpG, gpO, and gpP, whose molecular weights (MW) are estimated to be 64, 43, 29 and 26 kilodaltons (kDa), respectively. More than 16 minor proteins ranging from 113 to 9.6 kDa were also detected. The genes for the major capsid proteins were cloned and each DNA sequence was determined. N-terminal amino acid alignments determined by protein sequencing completely coincided with those deduced from the nucleotide sequences.